retroviral vector pmscv (Addgene inc)
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95
Structured Review
Addgene inc
retroviral vector pmscv
Retroviral Vector Pmscv, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmscv+retroviral+vector/MSCV-IRES-GFP+(Plasmid+%2320672)/pmc12785862-166-31-39
Average 95 stars, based on 150 article reviews
Retroviral Vector Pmscv, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmscv+retroviral+vector/MSCV-IRES-GFP+(Plasmid+%2320672)/pmc12785862-166-31-39
Average 95 stars, based on 150 article reviews
retroviral vector pmscv - by Bioz Stars,
2026-10
95/100 stars
Images
1) Product Images from "Pre-Treatment with Dacarbazine Sensitizes B16 Melanoma to CAR T Cell Therapy in Syngeneic Mouse Model"
Article Title: Pre-Treatment with Dacarbazine Sensitizes B16 Melanoma to CAR T Cell Therapy in Syngeneic Mouse Model
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms27010189
Figure Legend Snippet: Design of human/murine hybrid epidermal growth factor receptor (EGFR) and EGFR murine chimeric antigen receptor (CAR). ( A ) Schematic of the human/murine hybrid EGFR with cetuximab-binding substitutions (G442S, R467K, M491I, N492S, K497N) and its engagement by a cetuximab-derived single-chain variable fragment (scFv) on mCAR T cells. ( B ) The retroviral vector encoding the EGFR-specific murine CAR and green fluorescent protein (GFP); I—domain I; II—domain II; III—domain III; IV—domain IV.
Techniques Used: Binding Assay, Derivative Assay, Retroviral, Plasmid Preparation
Figure Legend Snippet: Generation of murine EGFR CAR T cells. ( A ) Workflow for EGFR mCAR T cell production, including T cell isolation, activation, retroviral transduction, and subsequent in vitro and in vivo experiments created with BioRender ( https://www.biorender.com/ (accessed on 1 September 2025)). ( B ) Representative flow cytometry histogram showing the transduction efficiency of murine T cells with the EGFR-targeted murine CAR construct. ( C ) Summary of independent transduction preparations: percentage of GFP + cells in each preparation. ( D ) Expansion ( left ) and viability ( right ) of EGFR mCAR T cells compared to non-transduced T cells over 14 days of culture. * p < 0.05 and *** p < 0.001.
Techniques Used: Cell Isolation, Activation Assay, Retroviral, Transduction, In Vitro, In Vivo, Flow Cytometry, Construct
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MG50534-UT), cDNAs were subcloned into the Retroviral:Article Title: The volume regulated anion channel LRRC8C suppresses T cell function by regulating cyclic dinucleotide transport and STING-p53 signaling Article Snippet: .. For overexpression of murine LRRC8C (Dharmacon, MMM1013–202767571) and p53 (Sino Biological, MG50534-UT), cDNAs were subcloned into the Article Title: The volume-regulated anion channel LRRC8C suppresses T cell function by regulating cyclic dinucleotide transport and STING-p53 signaling. Article Snippet: For overexpression of murine LRRC8C (Dharmacon, catalog no. MMM1013202767571) and p53 (Sino Biological, catalog no. MG50534-UT), cDNAs were subcloned into the pMSCV retroviral vector (Addgene, catalog no. 52114); empty pMSCV was used as control. .. For overexpression of murine LRRC8C (Dharmacon, catalog no. MMM1013202767571) and p53 (Sino Biological, catalog no. 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For overexpression of murine LRRC8C (Dharmacon, MMM1013–202767571) and p53 (Sino Biological, MG50534-UT), cDNAs were subcloned into the Article Title: Oncoprotein SET-associated transcription factor ZBTB11 triggers lung cancer metastasis Article Snippet: .. Mammalian expression constructs, including SET, ZBTB11, MMP9, and PRRG2, were generated by cloning each cDNA into a modified pIRESneo2 vector (Clontech, 6938-1), a pCMV-myc vector (Clontech, 635689), an SFB-NT or -CT destination vector (Gifted by Dr. Wenqi Wang, University of California Irvine), a pBabe retrovirus vector (Addgene, 1764) or a Control:Article Title: The volume regulated anion channel LRRC8C suppresses T cell function by regulating cyclic dinucleotide transport and STING-p53 signaling Article Snippet: .. 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Mammalian expression constructs, including SET, ZBTB11, MMP9, and PRRG2, were generated by cloning each cDNA into a modified pIRESneo2 vector (Clontech, 6938-1), a pCMV-myc vector (Clontech, 635689), an SFB-NT or -CT destination vector (Gifted by Dr. Wenqi Wang, University of California Irvine), a pBabe retrovirus vector (Addgene, 1764) or a Construct:Article Title: Oncoprotein SET-associated transcription factor ZBTB11 triggers lung cancer metastasis Article Snippet: .. Mammalian expression constructs, including SET, ZBTB11, MMP9, and PRRG2, were generated by cloning each cDNA into a modified pIRESneo2 vector (Clontech, 6938-1), a pCMV-myc vector (Clontech, 635689), an SFB-NT or -CT destination vector (Gifted by Dr. Wenqi Wang, University of California Irvine), a pBabe retrovirus vector (Addgene, 1764) or a Generated:Article Title: Oncoprotein SET-associated transcription factor ZBTB11 triggers lung cancer metastasis Article Snippet: .. Mammalian expression constructs, including SET, ZBTB11, MMP9, and PRRG2, were generated by cloning each cDNA into a modified pIRESneo2 vector (Clontech, 6938-1), a pCMV-myc vector (Clontech, 635689), an SFB-NT or -CT destination vector (Gifted by Dr. Wenqi Wang, University of California Irvine), a pBabe retrovirus vector (Addgene, 1764) or a Cloning:Article Title: Oncoprotein SET-associated transcription factor ZBTB11 triggers lung cancer metastasis Article Snippet: .. Mammalian expression constructs, including SET, ZBTB11, MMP9, and PRRG2, were generated by cloning each cDNA into a modified pIRESneo2 vector (Clontech, 6938-1), a pCMV-myc vector (Clontech, 635689), an SFB-NT or -CT destination vector (Gifted by Dr. Wenqi Wang, University of California Irvine), a pBabe retrovirus vector (Addgene, 1764) or a Modification:Article Title: Oncoprotein SET-associated transcription factor ZBTB11 triggers lung cancer metastasis Article Snippet: .. Mammalian expression constructs, including SET, ZBTB11, MMP9, and PRRG2, were generated by cloning each cDNA into a modified pIRESneo2 vector (Clontech, 6938-1), a pCMV-myc vector (Clontech, 635689), an SFB-NT or -CT destination vector (Gifted by Dr. Wenqi Wang, University of California Irvine), a pBabe retrovirus vector (Addgene, 1764) or a Binding Assay:Article Title: Inhibiting migration of cancer cells Article Snippet: A vector containing Nnat coding sequence and 3′-UTR was purchased from Origene (Cat#SC126815, Origene, Md.). .. To generate Nnat-mut, the miR-708 binding site was mutated in the Nnat 3′-UTR and cloned it into the Clone Assay:Article Title: Inhibiting migration of cancer cells Article Snippet: A vector containing Nnat coding sequence and 3′-UTR was purchased from Origene (Cat#SC126815, Origene, Md.). .. To generate Nnat-mut, the miR-708 binding site was mutated in the Nnat 3′-UTR and cloned it into the |
